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| Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL) of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Aluminum; Lipid peroxidation; Chemiluminescence; Platelet aggregation. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500005 |
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| Matias,Mariana Santos; Sousa,Bruna Barbosa de; Pereira,Déborah Fernanda da Cunha; Dias,Edigar Henrique Vaz; Mamede,Carla Cristine Neves; Queiroz,Mayara Ribeiro de; Silva,Anielle Christine Almeida; Dantas,Noelio Oliveira; Soares,Andreimar Martins; Costa,Júnia de Oliveira; Oliveira,Fábio de. |
| Abstract Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom. Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Bothrops alternatus; DC protein; Platelet aggregation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100324 |
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| Rádis-Baptista,G.; Moreno,F. B. M. B.; Nogueira,L. L.; Martins,A. M. C.; Toyama,D. O.; Toyama,M. H.; Azevedo Jr,W. F.; Cavada,B. S.; Yamane,T.. |
| Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durisssus venom; Snake venom C-type lectin; Homology modeling; Platelet aggregation; Antimicrobial activity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 |
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| Camargo,L.M.; França,C.N.; Izar,M.C.; Bianco,H.T.; Lins,L.S.; Barbosa,S.P.; Pinheiro,L.F.; Fonseca,F.A.H.. |
| It is not known whether the addition of ezetimibe to statins adds cardiovascular protection beyond the expected changes in lipid levels. Subjects with coronary heart disease were treated with four consecutive 1-week courses of therapy (T) and evaluations. The courses were: T1, 100 mg aspirin alone; T2, 100 mg aspirin and 40 mg simvastatin/10 mg ezetimibe; T3, 40 mg simvastatin/10 mg ezetimibe, and 75 mg clopidogrel (300 mg initial loading dose); T4, 75 mg clopidogrel alone. Platelet aggregation was examined in whole blood. Endothelial microparticles (CD51), platelet microparticles (CD42/CD31), and endothelial progenitor cells (CD34/CD133; CDKDR/CD133, or CD34/KDR) were quantified by flow cytometry. Endothelial function was examined by flow-mediated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endothelial microparticles; Platelet microparticles; Platelet aggregation; Endothelial progenitor cells; Simvastatin/ezetimibe. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500432 |
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| Barbosa,PSF; Martins,AMC; Toyama,MH; Joazeiro,PP; Beriam,LOS; Fonteles,MC; Monteiro,HSA. |
| Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops moojeni; Kidney; Platelet aggregation; Insulin; Antibacterial activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016 |
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| More,SS; Kiran,KM; Veena,SM; Gadag,JR. |
| Snake venoms are rich in enzymes such as phospholipase A2, proteolytic enzymes, hyaluronidases and phosphodiesterases, which are well characterized. However, L-amino acid oxidase (LAO EC.1.4.3.2) from snake venoms has not been extensively studied. A novel L-amino acid oxidase from Bungarus caeruleus venom was purified to homogeneity using a combination of ion-exchange by DEAE-cellulose chromatography and gel filtration on Sephadex® G-100 column. The purified monomer of LAO showed a molecular mass of 55 ±1 kDa estimated by SDS-PAGE. The specific activity of purified LAO was 6,230 ± 178 U/min/mg, versus 230 ± 3.0 U/min/mg for the whole desiccated venom, suggesting a 27-fold purification with a 25% yield. Optimal pH and temperature for maximum purified enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-amino acid oxidase; Bungarus caeruleus; Platelet aggregation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100007 |
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